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Health technologies European and American Pharmacopoeia. Report of results will include an observation of zones of inhibition and growth under the specimen if present. The Parallel Streak Method has proven effective over a number of years of use in providing evidence of antibacterial activity against both Gram positive and Gram negative bacteria.
Avoid contamination by using good sterile technique in plating and transferring. Allow agar to gel firmly before inoculating. Other organisms can also be used depending on the intended end-use. The test Method determines antibacterial activity of diffusable antimicrobial agents on treated textile materials.
Some of the bacteria used in this test are pathogenic; i. Notes and References Precision and Bias 7. The method is useful for obtaining a rough estimate of activity in that the growth of the inoculum organism decreases from one end of each streak to the other and from one metgod to the next resulting in increasing degrees of sensitivity. Wear safety glasses in tfst laboratory areas. The size of the zone of inhibition and the narrowing of the streaks caused by the presence of the antibacterial agent permit an estimate of the residual antibacterial activity after multiple washings.
Therefore, every necessary and 5. Check pH and adjust to 7. The test is followed in the cases where the antibacterial agent is migratory i. These safety precautions are for information purposes only. This test method is subject to revision at any time by the responsible technical committee and must be reviewed every five years and if not revised, either reaffirmed or withdrawn.
A zone of inhibition occurs as a result of the diffusion of an antimicrobial agent from the specimen. After incubation, a clear area of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of the specimen. Avoid mutation by strict adherence to monthly stock transfers. Treated materials should be compared to an untreated corresponding material and a material specimen with known bacteriostatic activity. The precautions are ancillary to the testing procedures and are not intended to be all inclusive.
The hard copy print may only be distributed to other employees for their internal use within your organization. May be sterilized in 1, mL borosilicate glass flasks and petri dishes poured from this.
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